| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Holdemania filiformis | TCCAACTCCGGAAACCACG | TGTGCTTCATTGCCGTGC | 59.93 | 59.36 | 242 | 78 |
100.00%
|
50.00%
|
| Primer 222 | Holdemania filiformis | TGCGAACCGGAAATCTGGG | AGCGCACCGTGTTAAGCT | 60.38 | 59.97 | 235 | 77 |
100.00%
|
50.00%
|
| Primer 223 | Holdemania filiformis | ACGAGCTGACATTGGTGCA | TCCTCTGGCGAAATCGAGC | 59.93 | 59.86 | 289 | 76 |
100.00%
|
50.00%
|
| Primer 224 | Holdemania filiformis | AACGATGTGCCGTCCGTT | TCGCCAGCTGGAACAACA | 59.97 | 59.49 | 190 | 76 |
100.00%
|
50.00%
|
| Primer 225 | Holdemania filiformis | AACGATGTGCCGTCCGTT | TCCAATGTCCGACGCGAA | 59.97 | 59.35 | 263 | 76 |
100.00%
|
50.00%
|
| Primer 226 | Holdemania filiformis | AAAGGAACCACCGCTGACC | AGCGCTGACGGAAATCCT | 60.23 | 59.02 | 209 | 76 |
100.00%
|
50.00%
|
| Primer 227 | Holdemania filiformis | AACAACGCCTTGCTTGCC | TTTGCGGACAAGCCAGGA | 59.58 | 59.49 | 181 | 76 |
100.00%
|
50.00%
|
| Primer 228 | Holdemania filiformis | ACGCAAAGCAACGAACCG | TCCGTTTCCACACCGCAA | 59.67 | 59.81 | 254 | 76 |
100.00%
|
50.00%
|
| Primer 229 | Holdemania filiformis | TGATTGAGCGCTACGGCA | TCAGCGGACGCACAAACT | 59.42 | 59.89 | 162 | 76 |
100.00%
|
50.00%
|
| Primer 230 | Holdemania filiformis | TCGTCTCGGTGATCAGCAC | AAGCGGATGACCAGCGTT | 59.49 | 59.65 | 175 | 76 |
100.00%
|
50.00%
|